Pesquisa | SciELO

Precisa de ajuda?

Adicionar outro campo +

Histórico de busca

Não foram encontrados documentos para sua pesquisa

Resultados: 40

au:Espejo Espejo, José

Filtros

Coleções + Opções

Todos
Chile

10
Peru

9
Espanha

6
Brasil

4
Colômbia

3
Saúde Pública

3
Venezuela

3
México

2

Periódico + Opções

Todos
Arnaldoa

6
Latin american journal of aquatic research

4
Nefrología (Madrid)

4
Austral journal of veterinary sciences

2
Cadernos de Saúde Pública

2
Revista Colombiana de Psiquiatría

2
Revista Española de Salud Pública

2
Revista de Saúde Pública

2
Salud de los Trabajadores

2
Acta bioethica

1
Frontera norte

1
Gayana. Botánica

1
Idesia (Arica)

1
La ventana. Revista de estudios de género

1
Memórias do Instituto Oswaldo Cruz

1

Mostrar tudo...

Idioma

Todos
Português

4

Ano de publicação + Opções

Todos
2019

6
2017

4
2020

3
2021

3
1998

2
2003

2
2004

2
2008

2
2010

2
2013

2
2016

2
2018

2
2022

2
1989

1
2001

1

Mostrar tudo...

SciELO Áreas Temáticas + Opções

Todos
Ciências da Saúde

19
Ciências Biológicas

13
Ciências Humanas

5
Ciências Exatas e da Terra

4
Ciências Agrárias

3
Ciências Sociais Aplicadas

2
Multidisciplinar

1

WoS Áreas Temáticas + Opções

Todos
Biologia

10
Health

10
Sciences

10
Environmental

9
Occupational

7
Public

7
Policy

6
Services

6
Pesca

4
Freshwater

4
Marine

4
Multidisciplinary

4
Nephrology

4
Oceanografia

4
Urology

4

Mostrar tudo...

WoS Índice de Citações + Opções

Todos
Science Citation Index Expanded

17
Social Sciences Citation Index

7

Citáveis e não citáveis + Opções

Todos
Citável

37
Não citável

3

Tipo de literatura + Opções

Todos
Artigo

31
Comunicação rápida

4
Carta

2
Relato de caso

1
Artigo de revisão

1
Outros

1

Ordenar por

Página de 3 Próxima

Selecionar esta página

Imprimir | Enviar por e-mail | Exportar |

Facebook
Twitter
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

0 Itens selecionados

Listar itens
Apagar todos da lista

A global-level assessment of gulls (Larus spp.) as bioindicators of trace elements in coastal ecosystems
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Bahamonde, Paulina

; Espejo, Winfred

; Celis, José

; Montes, Isis-Yelena

; Barra, Ricardo O.

Latin american journal of aquatic research

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Mai 2023, Volume 51 Nº 2 Páginas 195 - 209

Resumo: En | Texto: En | PDF: En

ABSTRACT. Anthropogenic activities and the demand for trace elements have risen, causing an increase in their environmental levels, which could affect biota. High levels of trace elements in living beings have been associated with toxicity, metabolic disorders, oxidative stress, and cancer. Seabirds, such as gulls, have been used as bioindicators of environmental pollution caused by anthropogenic sources. Gulls are widely distributed worldwide, usually occupying high trophic levels, and living close to humans. Among gulls, Larus spp. are omnivorous, predominantly carnivorous, nest on the ground, and capture live food or steal it opportunistically. The present work summarizes the data of trace elements found in eggs, feathers, blood, and feces, among other internal tissues, of several gull species of the genus Larus around the world. Most of the reports are from the Northern Hemisphere (70%), particularly from Europe and North America. The elements Cd, Hg, Mn, Pb, and Se are the most studied (54%), among which Hg represents about 19%. On the other hand, there is no information for lesser-known trace elements such as Rb, Ti, new technology elements (e.g. Ge, Re, Ta), rare earth elements (e.g. Ce, La, Y), or elements of the platinum group (e.g. Os, Ir, Ru). Even though Larus spp. is a suitable bioindicator of chemical contamination in marine ecosystems, only 28 of the 53 species of the Larus genus have been used on trace elements pollution. Future research should address lesser-known elements which are increasingly used by new technologies.

https://doi.org/10.3856/vol51-issue2-fulltext-2977

Assessing the influence of Humboldt penguin (Spheniscus humboldti) by excrements on the levels of trace and rare earth elements in the soil
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Celis, José E.

; Espejo, Winfred

; Padilha, Janeide de A.

; Sandoval, Marco

Latin american journal of aquatic research

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Nov 2022, Volume 50 Nº 5 Páginas 782 - 789

Resumo: En | Texto: En | PDF: En

ABSTRACT. Rare earth elements (REE) and some transition metals (e.g. Nb) are a group of chemicals that have recently been widely used in industrial processes due to the increasing demand for new technologies. As a result, these chemicals are increasingly being released into the environment, which could mean that these pollutants could modify marine and terrestrial ecosystems. Seabirds, such as penguins, can biotransport pollutants and nutrients from the sea to land through excreta. However, there is no information about the role of the Humboldt penguin (Spheniscus humboldti) in bio-transporting emerging contaminants such as REE. This study aimed to assess any possible contribution of Humboldt penguins to the geochemical composition of some terrestrial areas. Excreta samples were collected from Chañaral Island, one of the most important sites in Chile for the conservation of Humboldt penguins. The results showed that this species tends to contribute to soil enrichment with REE (Ce, La, Nd, and Pr) and Nb through excreta, as well as with carbon. More studies are needed to see the potential impacts on the soil.

https://doi.org/10.3856/vol50-issue5-fulltext-2933

Efecto leishmanicida del extracto butanólico de Peperomia dolabriformis (Piperaceae)
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Meléndez Rodríguez, Deyvi

; Blas Cerdán, Willian

; Saldaña Jiménez, José

; Zavaleta Espejo, Gina

Arnaldoa

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jan 2022, Volume 29 Nº 1 Páginas 205 - 216

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract The aim was to evaluate the leishmanicidal effect of the butanolic extract of Peperomia dolabriformis (Piperaceae) on the viability of the Leishmania peruviana promastigotes strain LC-26. The extract was obtained using Soxhlet equipment with 70% diluted butanol solvent with Milli Q water. For each 100 g of pulverized sample was added 1 liter of solvent which was then evaporated in a gas extractor. The leishmanicidal effect was carried out in a single-phase culture at a concentration of 106 promastigotes/mL of L. peruviana. The negative control was phosphate buffer. The experimental groups of the butanolic extract of P. dolabriformis at concentrations of 0.009 mg/mL, 0.09 mg/mL and 0.9 mg/mL, respectively, were incubated at 27 °C for 24, 48, 72 and 96 hours. The leishmanicidal effect showed efficacy of 100% mortality at a concentration of 0.9 mg/mL.

Resumen En la presente investigación se evaluó el efecto leishmanicida del extracto butanólico de Peperomia dolabriformis (Piperaceae) “congona de zorro” sobre la viabilidad de los promastigotos de Leishmania peruviana cepa LC-26. El extracto se obtuvo usando un equipo Soxhlet con solvente butanol diluido al 70% con agua Milli Q. Por cada 100 g de muestra pulverizada se adicionó 1 litro de solvente que luego fue evaporado mediante una cabina extractora de gases. La evaluación del efecto leishmanicida se realizó en un cultivo monofásico con una concentración de 106 promastigotos/mL. El control negativo fue buffer fosfato. Los grupos experimentales con el extracto butánolico tuvieron concentraciones de 0,009 mg/mL, 0,09 mg/mL y 0,9 mg/mL, respectivamente y se incubaron a 27 °C, durante 24, 48, 72 y 96 horas. El efecto leishmanicida presentó una eficacia del 100% de mortalidad a la concentración de 0,9 mg/mL.

https://doi.org/10.22497/arnaldoa.291.29113

Germinación, crecimiento y desarrollo de Bulnesia chilensis Gay bajo diferentes tratamientos pregerminativos
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics

Pañitrur, Carolina

; Navarro, Johana

; Espejo, Ma. José

; Sandoval, Ana

Gayana. Botánica

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Dez 2021, Volume 78 Nº 2 Páginas 121 - 130

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Bulnesia chilensis Gay (Zygophyllaceae) is an endemic shrub from the Atacama and Coquimbo regions, the only member of the genus in Chilean flora. Despite its importance for the development of conservation actions, there are no reports on its propagation by seeds. The objective of the present investigation was to evaluate the germination response, growth, development, and survival of B. chilensis under different pregerminative treatments. To accomplish this objective, 12 treatments were carried out, which included chemical and mechanical scarification, as well as methods of soaking, disinfection and washing of the seeds. Treatments consisted of 5 replicates of 15 seeds were established at 20°C and 12:12 photoperiod. The final percentage and the germination rate were calculated, as well as aerial and root biomass of the seedlings emerged per treatment. At 2 months post-transplantation, height, phenological state and survival of the plants obtained were determined. Although the use of techniques such as chemical and mechanical scarification (micropyle cutting of the seeds) favors germination, they generate seedlings with less aerial and root growth, affecting their subsequent survival. On the other hand, with the use of simple treatments such as washing the seeds and soaking them in water with disinfection, it is possible to obtain about 80% germination, favoring the subsequent plant survival and growth. It is expected to improve the production practice of this species, especially focused on its use in restoration programs.

RESUMEN Bulnesia chilensis Gay (Zygophyllaceae) es un arbusto endémico de las regiones de Atacama y Coquimbo, único representante del género en la flora chilena. A pesar de su importancia para el desarrollo de acciones de conservación, no hay reportes sobre su propagación por semilla. El objetivo de la presente investigación fue evaluar la respuesta germinativa, crecimiento, desarrollo y sobrevivencia de B. chilensis bajo diferentes tratamientos pregerminativos. Para ello, se emplearon 12 tratamientos, que incluyeron escarificación química y mecánica, así como métodos de remojo, desinfección y lavado de las semillas. Un total de 5 réplicas de 15 semillas cada una fue instalada por tratamiento y establecidas a temperatura de 20°C y fotoperiodo 12:12. Se calculó el porcentaje final y la tasa de germinación, así como la biomasa aérea y radicular de las plántulas emergidas por tratamiento. A los 2 meses post-trasplante, se midió la altura, el estado fenológico y la sobrevivencia de las plantas obtenidas. Si bien el empleo de técnicas como la escarificación química y mecánica (incisión en micrópilo de las semillas) favorece la germinación, éstas generan plántulas con un menor crecimiento aéreo y radicular, afectando a su posterior sobrevivencia. Por otra parte, con el empleo de tratamientos simples como el lavado de semillas y el remojo en agua de éstas más desinfección, se logra obtener cerca de un 80% de germinación, favoreciendo además el crecimiento y sobrevivencia de las plantas. Se espera mejorar la práctica de producción de esta especie, especialmente enfocada en su uso en programas de restauración.

38 downloads

El mundo educativo y su entorno ante la pandemia del siglo XXI: Retos, responsabilidades e inclusión
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

del Pino Espejo, Maria José

; Viltalba, Karina

Revista Historia de la Educación Latinoamericana

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Dez 2021, Volume 23 Nº 37 Páginas 9 - 10

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

https://doi.org/10.19053/01227238.13907 33 downloads

In memoriam: a eulogy for Daniel González-Acuña, 1963-2020
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Muñoz-Leal, Sebastián

; Silva-De-La-Fuente, María Carolina

; Barros-Battesti, Darci Moraes

; Guglielmone, Alberto Alejandro

; Venzal, José Manuel

; Nava, Santiago

; Sepúlveda, María Soledad

; Tkach, Vasyl

; Kinsella, Mike

; Fuentes-Castillo, Danny

; Llanos-Soto, Sebastián

; Grandón-Ojeda, Alexandra

; Celis, José Enrique

; Espejo, Winfred

; Jara-Carrasco, Solange

; Azat, Claudio

; Martins, Thiago Fernandes

; Labruna, Marcelo Bahia

; Briceño, Cristóbal

; Figueroa, Ricardo Antonio

; Corales-Stappung, Soraya

; Moreno, Lucila

Revista Brasileira de Parasitologia Veterinária

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2021, Volume 30 Nº 1 elocation e000821

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1590/s1984-29612021005 279 downloads

Trace metal concentrations and body condition in adult Adélie penguins (Pygoscelis adeliae) from the western Antarctic Peninsula
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Espejo, Winfred

; Braz, Bernardo Ferreira

; Santelli, Ricardo

; Padilha, Janeide de A.

; Dorneles, Paulo

; Malm, Olaf

; Celis, Christopher A.

; Celis, José E.

Latin american journal of aquatic research

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Nov 2020, Volume 48 Nº 5 Páginas 886 - 894

Resumo: En | Texto: En | PDF: En

ABSTRACT Adélie penguins (Pygoscelis adeliae) are seabirds that live exclusively in Antarctica, one of the planet's last pristine areas. However, this remote region is experiencing a continuing expansion of human activities that may affect Antarctic fauna. Trace metals constitute a menace to seabirds because they can adversely affect their health. There is a lack of studies relating to metals' levels in feathers with morphological parameters of seabirds. Trace metal levels were measured in Adélie penguin feathers and their body condition through the relative condition factor (ReCF) in adult individuals from two South Shetland Islands locations and two from Graham Land. Consequently, we determined the levels of some metals in feathers to see any relationship with morphological parameters linked to the bird's health. Our results showed significant differences in metals among locations studied and a significant relationship between ReCF with Cu and Zn in one of the South Shetland Islands. Also, penguins exhibited a significantly lower weight. We found a positive correlation between non-essential with essential metals, indicating that Cu acts as a detoxifying agent for Cr, Cd, and Pb. In contrast, Se could be for V and Pb. Although the relationship between most metals with ReCF was not significant, some site-specific factors may be influencing it, whereas metals may be affecting the organisms at low biological levels. Molecular, biochemical, and genetic studies are required to elucidate this issue.

https://doi.org/10.3856/vol48-issue5-fulltext-2455 190 downloads

Toxicidad del decocto de cálices de Physalis peruviana L. (Solanaceae) “aguaymanto” sobre larvas del tercer estadio de Artemia salina
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Pedro Huaman, Juan J.

; Blas Cerdán, Willian G.

; Zavaleta Espejo, Gina G.

; Saldaña Jiménez, José A.

; Vásquez Villanueva, Katherine N.

; Hoyos Honorio, Rosmery A.

; Tafur Trujillo, Karen A.

; Rodriguez Seminario, Carmen E.

; Rodríguez Pesantes, Daiana L.

; Tisnado López, Zully K.

Arnaldoa

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Mai 2020, Volume 27 Nº 2 Páginas 561 - 570

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract On the present investigacion was determinated the toxicity of the of calyx decoction of Physalis peruviana L. (Solanaceae) “aguaymanto” in the Artemia salina model. The experimental design was completely randomized, it had eight treatments including the control: 0, 50, 250, 500, 2500, 5000, 12500 and 25000 ppm. of “aguaymanto” calyx decoction Physalis peruviana L.; each treatment with three repetitions. The experimental units were composed of 100 uL from each group and 10 nauplii from stage III, in Eppendorf vials of 2ml. After 24 hours, the number of dead larvae and the LD50 were determined using the free version R statistical program. The LD50 had a value of 10258.02 ppm of P. peruviana calyx decoct, after 24 hours of exposure to A. salina. The decoction evaluated has low toxicity on the larvae of the III larval stage.

Resumen En el presente trabajo se determinó la toxicidad del decocto de cálices de Physalis peruviana L. (Solanaceae) “aguaymanto” en el modelo Artemia salina. El diseño experimental fue completamente aleatorizado, tuvo 08 tratamientos incluyendo al control: 0, 50, 250, 500, 2500, 5000, 12500 y 25000 ppm. del decocto de cálices de “aguaymanto” Physalis peruviana L.; cada tratamiento con 03 repeticiones. Las unidades experimentales estuvieron compuestas por 100 uL de cada tratamiento y 10 nauplios del estadio III, en viales Eppendorf de 2ml. Después de 24 horas, se determinó el número total de larvas muertas y la DL50 utilizando el programa estadístico R versión libre. La DL50 tuvo un valor de 10258,02 ppm del decocto de cálices de P. peruviana, a las 24 horas de exposición sobre A. salina. El decocto evaluado tiene baja toxicidad sobre las larvas del III estadio larval.

https://doi.org/10.22497/arnaldoa.272.27208

Does protein content influences accumulation and biomagnification of tantalum in fishes and invertebrates of marine coastal environments?
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Ricciardi, Rocío

; Espejo, Winfred

; Barra, Ricardo

; Chiang, Gustavo

; Celis, José E.

Latin american journal of aquatic research

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Mai 2020, Volume 48 Nº 2 Páginas 336 - 341

Resumo: En | Texto: En | PDF: En

ABSTRACT Tantalum (Ta) is a rare transition metal widely used in the manufacture of new technologies such as computers, cell phones, solar panels and implants in biomedicine, and their use is continuously increasing. Most non-essential metals are immobilized in the organism through complexation with proteins, but it is not known if new-technology elements (such as Ta) present the same behavior. In aquatic environments, metals tend to concentrate in biota and some can be biomagnified through the trophic chain, even reaching humans. This study aimed to investigate any possible relationship between Ta and proteins. Invertebrates and fishes were collected from coastal marine ecosystems of northern Chile, Patagonia, and South Shetland Islands (Antarctic Peninsula). A direct positive relationship was found between Ta and total proteins throughout the food chain from marine ecosystems of the Patagonia and the Antarctic Peninsula area, revealing that proteins are probably the pathway by which Ta bioaccumulates and biomagnifies in macroinvertebrates and fish of pristine coastal marine environments. Our data suggest that site-specific factors (e.g., water temperature, pH, geography) may be influencing the environmental fate of Ta. Therefore, further studies are needed to understand the biological implications of this metal.

https://doi.org/10.3856/vol48-issue2-fulltext-2416 325 downloads

10.

Producción de Bacillus thuringiensis H-14 var. israelensis (Bacillaceae) mutante por acción del cloruro de hidroxilamina
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Pedro Huaman, Juan J

; Blas Cerdán, Willian G

; Zavaleta Espejo, Gina G

; Saldaña Jiménez, José A

; Vasquez Villanueva, Katherine N

; Hoyos Honorio, Rosmery A

; Pedro Huaman, Liz S. R

Arnaldoa

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Set 2019, Volume 26 Nº 3 Páginas 1143 - 1152

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

In the present work, the production of mutant Bacillus thuringiensis H-14 var. israelensis (Bacillaceae) by the action of hydroxylamine chloride, was evaluated. The experimental design was completely randomized, with a control and three mutagen concentrations 10, 20 and 30 ppm with three repetitions for each. The bioprocess was carried out using agitated and aerated cylindrical tank bioreactors, with a microbial inoculum of 10(8) CFU / ml for 48 hours at a temperature of 30 ° C and an initial pH of 7,0, which decreased to a final value of 6,0. Obtaining the highest production of bioinsecticide at the concentration of 20 ppm. Which was quantified in grams of dry weight per liter (2,10 g / L). Evidence that there is no direct relationship between the production of bioinsecticide and the concentration of the mutagen.

En el presente trabajo se evaluó la producción de Bacillus thuringiensis H-14 var. israelensis (Bacillaceae) mutante por acción del cloruro de hidroxilamina. El diseño experimental que se realizó fue completamente al azar, con un testigo y tres concentraciones del mutágeno 10, 20 y 30 ppm con tres repeticiones para cada una. El bioproceso, se llevó a cabo empleando biorreactores tipo tanque cilíndrico aireado y agitado, con un inóculo microbiano de 10(8) UFC/ml durante 48 horas a temperatura de 30°C y un pH inicial de 7,0, el cual disminuyo hasta un valor final de 6,0. Obteniéndose la mayor producción de bioinsecticida a la concentración de 20 ppm. La cual fue cuantificada en gramos de peso seco por litro (2,10 g/L). Evidenciándose que no existe una relación directa entre la producción de bioinsecticida y la concentración del mutágeno.

https://doi.org/10.22497/arnaldoa.263.26319

11.

Efecto in vitro de extractos hidroalcohólicos de Mangifera indica, Tamarindus indica y Cassia angustifolia sobre el crecimiento de Salmonella typhi y Escherichia coli
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Untol Pa, Roxana

; Zavaleta Espejo, Gina

; Saldaña Jiménez, José

; Blas Cerdán, Willian

Arnaldoa

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Mai 2019, Volume 26 Nº 2 Páginas 713 - 724

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

In vitro effect of hydroalcoholic extracts of Mangifera indica, Tamarindus indica and Cassia angustifolia on the growth of Salmonella typhi and Escherichia coli ATCC 25922 was evaluated using a completely randomized factorial design. Hydroalcoholic extracts were obtained from the leaves of M. indica; T. indica and C. angustifolia using Soxhlet equipment with 90% alcohol, 50% and 25% concentrations were used for each extract and a mix at 50% and 25% of the three extracts. To evaluate the effect on bacterial growth the Kirby-Bauer method was used with 0.7 mm diameter Watman no. 4 paper discs, five discs were placed per plate for each concentration (extracts and the mix plus the positive control (ciprofloxacin)), incubating at 37 ºC for 24 hours, then the diameter of the inhibition zones was measured. The results show significant differences between the effect of the extracts and greater effect with the extract of M. indica with an inhibition halo of 16.53 mm for S. typhi and 15.16 for E. coli at the 50% concentration,; this would be possible due to mangiferin, a polyphenol that would produce growth inhibition.

Se evaluó el efecto in vitro de extractos hidroalcohólicos de Mangifera indica, Tamarindus indica y Cassia angustifolia sobre el crecimiento de Salmonella typhi y Escherichia coli ATCC 25922 usando un diseño factorial completamente al azar. Los extractos hidroalcohólicos se obtuvieron de las hojas de M. indica, T. indica y C. angustifolia utilizando un equipo Soxhlet con alcohol al 90%, se usaron concentraciones al 50% y 25% para cada uno de los extractos y un mix al 50% y 25% de los tres extractos. Para evaluar el efecto sobre el crecimiento se empleó el método Kirby-Bauer con discos de papel Watman n° 4 de 0,7 mm de diámetro, se colocaron 5 discos por placa para cada concentración (extractos y el mix más el control positivo (ciprofloxacina)), incubándose a 37 ºC durante 24 horas, luego se midió el diámetro de las zonas de inhibición. Los resultados muestran diferencias significativas entre el efecto de los extractos, teniendo mayor efecto el extracto de M. indica con un halo de inhibición de 16,53 mm para S. typhi y de 15,16 para E. coli a la concentración de 50%; esto se debería probablemente a la mangiferina, un polifenol que produciría la inhibición del crecimiento.

https://doi.org/10.22497/arnaldoa.262.26213

12.

Efecto del extracto hidroalcohólico de Caesalpinia spinosa (Fabaceae) sobre el crecimiento de Salmonella typhi y Escherichia coli
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Cholán Pacheco, Karina

; Zavaleta Espejo, Gina

; Saldaña Jiménez, José

; Blas Cerdán, Willian

Arnaldoa

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Mai 2019, Volume 26 Nº 2 Páginas 699 - 712

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

In the present investigation, the effect of the hydroalcoholic extract of Caesalpinia spinosa (Fabaceae) on the growth of Salmonella typhi and Escherichia coli was determined using a completely randomized block design. For which, a hydroalcoholic extract was prepared at 70° with mature and dry pods of C. spinosa. The evaluation was carried out using the modified Kirby Bauer method; instead of using disks, holes were used made with a sterile steel punch with a diameter of 7 mm. In each of the holes, 50 uL of the hydroalcoholic extract of C. spinosa was placed at concentrations of 200 mg/ml; 400 mg/ml and 800 mg/ml respectively; a positive control (ciprofloxacin) and a negative control (2 % dimethyl sulfoxide) were used, incubation at 37 ° C for 24 hours and finally the diameters (mm) of the inhibition zones were measured. The results show a directly proportional relationship between the increase in the concentration of the extract and the inhibition of the growth of both bacteria. It is observed that S. typhi has the largest inhibition diameter (29.33 mm) at the concentration of 800 mg/ml, while E. coli has an inhibition diameter of 24.44 mm at the same concentration. This is probably due to the antibacterial action of compounds such as tannins, quinones, phenols and flavonoids, gallotannins and gallic acid present in the extract. It is concluded that the hydroalcoholic extract of C. spinosa at different concentrations inhibits the growth of Salmonella typhi and Escherichia coli under laboratory conditions

En la presente investigación se determinó el efecto del extracto hidroalcohólico de Caesalpinia spinosa (Fabaceae) sobre el crecimiento de Salmonella typhi y Escherichia coli usando un diseño en bloques completamente al azar. Para lo cual, se preparó un extracto hidroalcohólico al 70° con vainas maduras y secas de C. spinosa. La evaluación se realizó por el método de Kirby Bauer modificado; en lugar de usar discos se usaron hoyos realizados con un sacabocado estéril de acero de 7 mm de diámetro; en cada uno de los hoyos se colocó 50 uL del extracto hicroalcohólico de C. spinosa a las concentraciones de 200 mg/ml; 400 mg/ml y 800 mg/ml respectivamente; se trabajó con un control positivo (ciprofloxacino) y un control negativo (dimetilsulfoxido al 2 %), incubación a 37 ºC durante 24 horas y finalmente se midieron los diámetros (mm) de los halos de inhibición. Los resultados hallados evidencian una relación directamente proporcional entre el incremento de la concentración del extracto y la inhibición del crecimiento de ambas bacterias. Observándose que S. typhi presenta el mayor diámetro de inhibición (29,33 mm) a la concentración de 800 mg/ml, mientras que E. coli presenta un diámetro de inhibición de 24,44 mm a la misma concentración. Lo cual probablemente se debe a la acción antibacteriana de compuestos como taninos, quinonas, fenoles y flavonoides, galotaninos y acido gálico presentes en el extracto. Se concluye que el extracto hidroalcohólico de C. spinosa a diferentes concentraciones inhibe el crecimiento de Salmonella typhi y Escherichia coli en condiciones de laboratorio

https://doi.org/10.22497/arnaldoa.262.26212

13.

Identificación genotípica de Staphylococcus con fenotipo meticilino resistente aislados de muestras de humanos, animales y ambiente
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Espejo, Luis José

; Rodríguez, Karen Lorena

; Rodríguez, Martha Fabiola

; Gómez Ramírez, Arlen Patricia

Revista de Investigaciones Veterinarias del Perú

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jan 2019, Volume 30 Nº 1 Páginas 364 - 376

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

The aim of this study was to identify the mecA-1 gene of Staphylococcus isolates with methicillin-resistant phenotype (SMR) obtained from biological samples and surfaces of a veterinary clinic. Nine isolates with SMR phenotype classified by the automated VITEK system were selected. The genus and the variability were corroborated by amplification and sequencing of the 16S rRNA and tuf genes. The presence of the mecA1 gene was also established by PCR. In the nine isolates with SMR phenotype, the 16S rRNAand tuf genes were identified, showing 99% similarity with reference strains; however, only in five isolates was found the mecA-1 gene. The results showed a greater identification of the MR genotype in intra-hospital surfaces, which indicates the need for control and monitoring of these strains in veterinary hospitals due to the implications they have on public health.

El objetivo del estudio fue dentificar el gen mecA-1 de aislados de Staphylococcus con fenotipo meticilino resistente (SMR) obtenidos de muestras biológicas y superficies de una clínica veterinaria. Se seleccionaron nueve aislamientos con fenotipo SMR clasificado por el sistema automatizado VITEK. El género y la variabilidad se corroboraron por amplificación y secuenciación de los genes 16S rRNA y tuf. También se estableció la presencia del gen mecA-1 por PCR. En los nueve aislamientos con fenotipo SMR se identificaron los genes 16S rRNA y tuf, evidenciando un 99% de similitud con cepas de referencia; sin embargo, solo en cinco muestras se encontró el gen mecA-1. Los resultados demuestran una mayor identificación del genotipo MR en superficies intrahospitalarias, lo que evidencia la necesidad del control y seguimiento de estas cepas en hospitales veterinarios debido a las implicaciones que tienen en salud pública.

https://doi.org/10.15381/rivep.v30i1.14614

14.

Efecto antibacteriano de nanopartículas de ZnO sobre Staphylococcus aureus y Salmonella typhi
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Zavaleta Espejo, Gina

; Saldaña Jiménez, José

; Jáuregui Rosas, Segundo R

; Pacherrez Gallardo, Diego

; Rivera Burgos, María

; Samanamud Moreno, Fanny V

; Perales Pérez, Oscar J

Arnaldoa

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jan 2019, Volume 26 Nº 1 Páginas 421 - 430

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

The antibacterial effect of ZnO nanoparticles, obtained by the modified sol-gel method, on Staphylococcus aureus ATCC 25923 and Salmonella typhi was evaluated, using the modified KirbyBauer method with 7 mm diameter holes made with sterile punches instead of discs. A completely randomized block experimental design was developed with four treatments and a negative control (PEG 6000) with five repetitions. The concentrations of ZnO nanoparticles (0.4, 0.8, 1.2, 1.6, 2.0 mg/ ml) were prepared in aqueous solutions of PEG 6000 at the concentration of 100 mg/ml. The results obtained show a relationship between the increase in the concentration of the nanoparticles and the antibacterial effect, noticing the highest growth inhibition halo (14.78 mm) of S. aureus at the concentration of 2.0 mg/ml, unlike S. typhi, which had a smaller diameter of inhibition (11.01 mm) at the same concentration. This is probably because the nanoparticles generate free radicals (ROS), which when interacting with the bacterial cell wall cause an extrusion of the intracellular content due to the oxidative damage of proteins and lipids, generating in this way an antibacterial effect. Due to this, it is presumed that in the future they could become an alternative to fight bacterial diseases.

El efecto antibacteriano de nanopartículas de ZnO, obtenidas por el método sol-gel modificado, sobre Staphylococcus aureus ATCC 25923 y Salmonella typhi fue evaluado empleando el método Kirby-Bauer modificado con hoyos de 7 mm de diámetro realizados con sacabocados estériles en lugar de usar discos. Se desarrolló un diseño experimental en bloques completamente al azar con cuatro tratamientos y un control negativo (PEG 6000) con cinco repeticiones. Las concentraciones de nanopartículas de ZnO (0.4, 0.8, 1.2, 1.6, 2.0 mg/ml) fueron preparadas en soluciones acuosas de PEG 6000 a la concentración de 100 mg/ml. Los resultados obtenidos muestran una relación entre el incremento en la concentración de las nanopartículas y el efecto antibacteriano, notándose el mayor halo de inhibición del crecimiento (14.78 mm) para S. aureus a la concentración de 2.0 mg/ ml, a diferencia de S. typhi que presentó un menor diámetro de inhibición (11.01 mm) a la misma concentración. Lo cual probablemente se debería a que las nanopartículas generan radicales libres (ROS), que al interactuar con la pared celular bacteriana ocasionan una extrusión del contenido intracelular debido al daño oxidativo de proteínas y lípidos, originando un efecto antibacteriano. Debido a esto se presume que en un futuro podrían convertirse en una alternativa para combatir enfermedades bacterianas.

https://doi.org/10.22497/arnaldoa.261.26122

15.

Report of 2018 equine influenza outbreak in Chile
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics

Moreira, Rubén

; García, Alfonso

; Ahumada, Carolyn

; Badía, Catalina

; Suárez, Paulina

; Yangari, Betty

; Aguayo, Carolina

; Herrera, José

; Espejo, Gabriela

; Pinto, Enrique

Austral journal of veterinary sciences

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jan 2019, Volume 51 Nº 1

Resumo: En | Texto: En | PDF: En

Abstract: The present study reports an outbreak of equine influenza (EI) occurred in Chile in January 2018, with cases spread along the country, from the Atacama to the Magallanes Region. The virus identified corresponded to the H3N8 subtype, affecting equines, donkeys, and mules. Clinical signs ranged from mild to severe, with a higher mortality rate in donkeys.

1912 downloads

Exibindo

itens por página

Página de 3 Próxima

Selecione um ou mais resultados

Todos Ordem alfabética

Resultados

Gráfico comparativo Download CSV

Visualizar estatísticas de

Enviar resultado

E-mail do(s) destinatários*

Utilize ; (ponto e vírgula) para mais de um e-mail.

Enviar por e-mail

Esta página

Registros selecionados

Todos os registros (max. 300)

Alterar remetente, assunto e comentários

Seu email

Assunto

Comentários

Alterar remetente, assunto e comentários

Exportar resultados

Formato de exportação:

RIS (para EndNote, ProCite, Reference Manager, RefWorks, Citavi, etc.)

BibTeX

Citação

CSV (para Excel, etc)

Exportar:

Esta página

Itens selecionados

Todos os registros (max. 2000)

Mostrar mais

Sem resultados

Não foram encontrados documentos para sua pesquisa

Glossário e ajuda para busca

Você pode enriquecer sua busca de uma forma muito simples. Use os índices de pesquisa combinados com os conectores (AND ou OR) e especifique cada vez mais sua busca.

Por exemplo, se você deseja buscar artigos sobre
casos de dengue no Brasil em 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

Veja abaixo a lista completa de índices de pesquisa que podem ser usados:

Cód. do Índice	Elemento
ti	título do artigo
au	autor
kw	palavras-chave do artigo
subject	assunto (palavras do título, resumo e palavras-chave)
ab	resumo
ta	título abreviado da revista (ex. Cad. Saúde Pública)
journal_title	título completo da revista (ex. Cadernos de Saúde Pública)
la	código do idioma da publicação (ex. pt - Português, es - Espanhol)
type	tipo do documento
pid	identificador da publicação
publication_year	ano de publicação do artigo
sponsor	financiador
aff_country	código do país de afiliação do autor
aff_institution	instituição de afiliação do autor
volume	volume do artigo
issue	número do artigo
elocation	elocation
doi	número DOI
issn	ISSN da revista
in	código da coleção SciELO (ex. scl - Brasil, col - Colômbia)
use_license	código da licença de uso do artigo

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
Email: scielo@scielo.org

Todo o conteúdo de www.scielo.org, exceto onde está identificado, está licenciado sob uma Licença Creative Commons.
saiba mais »